16s –

Chen G, Zeng R, Wang X, et al. Antithrombotic Activity of Heparinoid G2 and Its Derivatives from the Clam Coelomactra antiquata. Mar Drugs. 2022, Jan 5;20(1):50. Sun D, Wang C, Sun L, et al. Gooneratne R. Preliminary Report …

, - WeGene ...

, - pe150,, Clean reads 1335190186 Clean bases () 133519.02 Mapping rate (%) 90.39 Unique rate (%) 91.92 Dup...

PE 250|

PE150×250 PE150×250PE150*250150×250、≤24㎏;,,;,,,、、、、、、、、 …

PE150 -

:PE150*1200 22:41:00 : : PE150*1200? :PE450*800 ... PE150×750(),:,,...

: 20-60G/run/flow slide,18 flow slide /run: 9 days : 99.999% (DNA): SNP、Indel、SV、CNV :,。0911WGS$1726 (45×),$8005 (87×) :

400*600

: 20:12:44 【PE150*250400*600 PE150*250400*600,PE150*250400*600,

011126 ; 【】 【】 :,、,,,,,, …,

- - :

2014,, ... Illumina PE150 350bp ≥100 X Illumina PE150 350bp ≥30 X ...

("",)(Real Time)、(Whole Picture)(Life Long)。 ...

(RNA-Seq)-- ...

15-20 798 900 1000 mRNA,PE150,6Gb(40M Reads), (*.fastq) T0708 RNA-Seq 5-10 200 250 300 RNA-Seq,T0707, T0730

, - ...

,,sanger,、。,,Roche454、Illumina、Thermo ...

2021

HiSeq X PE150 lane, NovaSeq S4 PE150 lane () 117632 65 (MPW) 117324 66

PE150 250B

PE150 250B. pe150. --,,,、、、、、。. pe-i--100×150 ...

_ ()

20 PE150 HiSeq X ≥10ug 90% 85% 20 PE150 HiSeq 4000 ≥10ng 95% 90% 20 de novo PE150 3-10Kbp HiSeq 4000 PacBio RS II ≥30ug 95% 90% 30 ...

—illumina -

—illumina. -illumina. -illumina. :. 、sample prep():(). 、cluster generaton():(flowcell),PCR, ...

Hiseq4000,PE150 :10 Gb/ :20 Gb/ circRNAceRNA (PTB),。

/ - ...

﹣20℃ 10-20mg 2-3ml 4ml HiSeq X ten PE150 NovaSeq 6000 PE150 35 Campbell J D, Alexandrov A, Kim J, et al. Distinct patterns of somatic genome alterations in lung ...

____

HiSeq X ten PE150 NovaSeq 6000 8 Day SE, Coletta RL, Kim JY, etal. Next-generation sequencing methylation profiling of subjects with obesity identifiesnovel gene changes[J]. Clinical Epigenetics, 2016, 8(1): 1.